<p>I opened flasks of sterilized broth in the cellar of the
Paris observatory, where the air was still. Only one flask
out of ten became putrid, whereas eleven flasks out of
eleven opened in the courtyard quickly acquired a rich
growth of bacteria. I journeyed to Mt. Montanvert in the
Alps, where I opened twenty flasks of sterilized broth. Only
one became putrid. I concluded that the air in the cellar and
the air above the glacier were freer of bacteria than the air
in the city streets. But my adversaries performed similar
experiments with different results. Perhaps they were not
careful to follow my procedures. The neck of the flask must
be heated first to kill the bacteria on the glass; then a heated
instrument must be used to break the tip of the flask as it is
held high above the head. Immediately thereafter the flask
must be sealed again in a flame [Pasteur demonstrates the
procedure]. In these difficult researches, while I sternly
object to frivolous contradictions, I feel nothing but grat55
itude toward those who warn me if I should be in error.
I then devised a conclusive experiment. I boiled a nutritious
infusion in a flask with a long curved neck like this
one. The tip of the neck was not sealed but left open to the
outside air. Thus, there was no hindrance to the entrance of
fresh air with its vital force as claimed by the advocates
of spontaneous generation. But bacteria in the entering air
would be trapped by the walls of the long glass tube. The
fluid remained sterile so long as the flask was maintained
in the vertical position. If, however, I contaminated the
65 broth by allowing some of it to flow into the neck and then
back into the flask, putrefaction promptly followed. So we
see that life does not arise spontaneously. Life comes only
from life.</p>
<ol>
<li>The conclusive experiment (line 56) performed by
Pasteur was designed to answer critics who argued that:
A) the apparatus used in Pasteurs earlier experiments had not been adeu.quately sterilized
B) Pasteurs experiments related to spontaneous generation had no immediate application
C) the results of Pasteurs experiments in the Alps and in the cellar could not be replicated
D) the broth in the flasks of Pasteurs earlier experiments was not nutritious enough
Should have chosen<br>
E) heating made the air in the flasks of the earlier experiments unfit for spontaneous generation</li>
</ol>
<p>I choose C because Pasteur did say that “but my adversaries performed similar experiments with different results”. But the answer is E. Can you give me an explanation? Thank you.</p>
<p>The original test is here in case you need extra practice: <a href=“http://www.whw.name/Resources/Test_12.pdf[/url]”>http://www.whw.name/Resources/Test_12.pdf</a></p>