DNA electrophoresis

<p>We did a lab in bio that involved DNA electrophoresis (like a crime scene lab). In the first lane, we had a DNA size marker, in the second lane, we had a crime scene DNA, in the 3-7 lane, we had 5 suspects’ dna. After the electrophoresis, we measured the distance migrated. He wanted us to make a graph where the y axis is distance migrated in Cm and the x axis is the log base pair length… On that axis the quantities range from 200-40000… Can anyone tell me what those mean and how to graph them, cuz my teacher did not really explain them well</p>

<p>Log base pair length is how many nucleotides there are. A T C and G. So a 200 length one will have 200 pairs of those. To graph them, divide the max (40,000) into equal parts, make that your graph’s interval, and put the points down.</p>

<p>^So for all the strands on the gel, they all have equal nucleotide base pairs?</p>

<p>Each band on the gel contains all the DNA fragments of a certain length, and the distance the band traveled is related to the base-pair length of the DNA it contains (smaller fragments travel farther).</p>